α-Ketoglutarate Assay Kit - sufficient for 100colorimetricorfluorometrictests, high purity

Item Number

K486303

• Specifications & Purity: 200 Tests
• Storage Temp: Protected from light,Store at -20°C
• Shipped In: Ice chest + Ice pads

Product Description

Alpha ketoglutarate (α - KG) is a key intermediate in the tricarboxylic acid (TCA) cycle, formed by the oxidative degreasing of isocitrate by isocitrate dehydrogenase. The synthesis of α - KG from glutamate catalyzed by glutamate dehydrogenase is a key step in the anaerobic reaction of the TCA cycle. α - KG is an important nitrogen transporter protein. This alpha ketoglutarate assay kit provides a simple, sensitive, and rapid method for quantifying alpha KG in various samples.
Detection method: colorimetric method
Component

Component	Storage	Amount
Component A: Amplite™ Red	Freeze (<-15 °C), Minimize light exposure	1 vial
Component B1: Enzyme Mix 1	Freeze (<-15 °C), Minimize light exposure	2 bottles (lyophilized powder)
Component B2: Enzyme Mix 2	Freeze (<-15 °C), Minimize light exposure	2 vials (lyophilized powder)
Component C: Assay Buffer	Freeze (<-15 °C)	1 bottle (10 mL)
Component D: α-Ketoglutarate Standard	Freeze (<-15 °C), Minimize light exposure	10 mM (100 µL)
Component E: DMSO	Freeze (<-15 °C)	1 vial (100 µL)

Application
The alpha ketoglutarate assay kit has been used to determine the activity of alpha ketoglutarate.
Applicability
Suitable for determining a-ketoglutarate in various biological samples such as cell and tissue lysates

Principle

α-KG concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm) product, proportional to the α-KG present. Typical sensitivities of detection for this kit are 0.01 to 10 nmoles.