5-DBCO-PEG₄-dUTP, 10mM Aqueous Solution

Item Number

D487788

• Synonyms: 5-DBCO-PEG₄-dUTP
• Specifications & Purity: ≥95%, 10mM solution in 100 mM Tris-HCl
• Storage Temp: Store at -20°C
• Shipped In: Ice chest + Ice pads

Product Description

5-DBCO-PEG4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting DBCO-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-free click chemistry that offers the choice
to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks
to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging
to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins

Applications:
Incorporation into DNA/cDNA by
PCR with Taq polymerase in-house data

pH: 7.5 ±0.5
Spectroscopic Properties: λmax 289 nm, ε 12.5 L mmol-1 cm-1 (Tris-HCl pH 7.5)