20 hydroxyprogesterone, scientifically known as (20S) -17,20-dihydroxypregne-4-en-3-one, is a naturally synthesized steroid hormone in the adrenal cortex, ovaries, and placenta. In the female reproductive system, it plays a crucial role as an essential hormone. Determining the level of 20 hydroxyprogesterone is helpful for the diagnosis and treatment of various diseases. In addition, researchers have used (20S) -17,20-dihydroxypregne-4-en-3-one to investigate the effects of progesterone on carbohydrate, lipid, and protein metabolism.